CXT 350 and CXT 353 are semi-automated benchtop instruments for the frozen aliquotting of a wide range of sample types, including tissue, feces, serum, plasma, whole blood, urine, and other biofluids. The instruments extract multiple frozen cores or aliquots from a frozen sample. Parent sample and each frozen aliquot are maintained from -80°C to -150°C throughout the frozen aliquotting process via LN2 chilling, eliminating damaging freeze-thaw cycling. Features a compact benchtop design, user-friendly touch screen operation, and compatibility with a wide range of vial types. Single-use probes prevent sample-to-sample carryover and contamination. The laser-targeting feature enables coring of specific tissue sections, streamlining tissue processing workflows. Frozen aliquotting of fecal samples reduces sample handling and enables processing for a wide range of analyses. Frozen sample aliquotting also enables the distribution of smaller sample volumes (frozen aliquots), extending the use of critical samples, such as rare disease, late-stage disease, and pediatric samples.